RT Journal Article
SR Electronic
T1 Molecular determinations of <em>EGFR</em> and <em>EML4-ALK</em> on a single slide of NSCLC tissue
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 708
OP 710
DO 10.1136/jclinpath-2013-201502
VO 66
IS 8
A1 Ulivi, Paola
A1 Puccetti, Maurizio
A1 Capelli, Laura
A1 Chiadini, Elisa
A1 Bravaccini, Sara
A1 Calistri, Daniele
A1 Zoli, Wainer
A1 Amadori, Dino
A1 Candoli, Piero
YR 2013
UL http://jcp.bmj.com/content/66/8/708.abstract
AB Introduction Tyrosine kinase inhibitors (TKIs) and anti-anaplastic lymphoma kinase (ALK) agents are highly effective for the treatment of non-small cell lung cancer (NSCLC) patients harbouring specific alterations, and molecular characterisation of the tumour is needed even when limited tumour material is available. Methods 20 patients with a known epidermal growth factor receptor (EGFR) gene status were enrolled: 10 had mutated and 10 had wild type tumours. FISH analysis was performed on one cytological or histological sample to determine EML4-ALK status, after which the same cells scraped off each slide were used to evaluate the EGFR status. Results In the 10 EGFR mutated patients, molecular analysis showed the same results as those obtained before the FISH test. One patient with an EGFR mutation also showed an EML4-ALK translocation, and both FISH-positive and FISH-negative cells maintained the EGFR mutation. Conclusions EGFR mutation analysis can be performed on the same sample previously submitted to the EML4-ALK FISH procedure.