PT  - JOURNAL ARTICLE
AU  - von Rosenstiel, Charlotte
AU  - Wiestler, Benedikt
AU  - Haller, Bernhard
AU  - Schmidt-Graf, Friederike
AU  - Gempt, Jens
AU  - Bettstetter, Marcus
AU  - Rihani, Laura
AU  - Wu, Wei
AU  - Meyer, Bernhard
AU  - Schlegel, Jürgen
AU  - Liesche-Starnecker, Friederike
TI  - Correlation of the quantitative level of MGMT promoter methylation and overall survival in primary diagnosed glioblastomas using the quantitative MethyQESD method
AID  - 10.1136/jclinpath-2019-206104
DP  - 2020 Feb 01
TA  - Journal of Clinical Pathology
PG  - 112--115
VI  - 73
IP  - 2
4099  - http://jcp.bmj.com/content/73/2/112.short
4100  - http://jcp.bmj.com/content/73/2/112.full
SO  - J Clin Pathol2020 Feb 01; 73
AB  - Aims O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter methylation is a high predictive factor for therapy results of temozolomide in patients with glioma. The objective of this work was to analyse the impact of MGMT promoter methylation in patients with primary diagnosed glioblastoma (GBM) relating to survival using a quantitative method (methylation quantification of endonuclease-resistant DNA, MethyQESD) by verifying a cut-off point for MGMT methylation provided by the literature (</≥10%) and calculating an optimal cut-off.Methods 67 patients aged 70 years or younger, operated between January 2013 and December 2015, with newly diagnosed IDH wild-type GBM and clinical follow-up were retrospectively investigated in this study. A known MGMT promoter methylation status was the inclusion criteria.Results Median overall survival (OS) was 16.9 months. Patients who had a methylated MGMT promoter region of ≥10% had an improved OS compared with patients with a methylated promoter region of <10% (p=0.002). Optimal cut-off point for MGMT promoter methylation was 11.7% (p=0.012).Conclusion The results confirm that the quantitative level of MGMT promoter methylation is a positive prognostic factor in newly diagnosed patients with GBM. The cut-off provided by the literature (</≥10%) and the calculated optimal cut-off value of 11.7% give a statistically significant separation. Hence, MethyQESD is a reliable method to calculate MGMT promoter methylation in GBM.