Primer sequences used for PCR by target
| Target | Forward primer | Reverse primer |
| CALR exon 9 | gcagagaaacaaatgaaggacaaac | AGGAAACAGCTATGACCATcttcctccttgtcctcctca |
| JAK2 exon 12 | ccaacctcaccaacattacagag | AGGAAACAGCTATGACCATtccaatgtcacatgaatgtaaatcaa |
| JAK2 exon 13 | AGGAAACAGCTATGACCATctcttgaagaatgaaagccttgg | gtttctgtgtgctttatccagaact |
| JAK2 exon 14 | AGGAAACAGCTATGACCATtttctttgaagcagcaagtatg | agatgctctgagaaaggcatta |
| MPL exon 10 | AGGAAACAGCTATGACCATtagcctggatctccttggtg | gcggtacctgtagtgtgcag |