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Original research
Expression of MMR system genes is correlated to NF-kB in patients with oral squamous cell carcinoma
  1. Alexandre Fonseca1,
  2. Matheus Perez1,
  3. Glaucia Veiga1,
  4. Fábio Prosdócimi2,
  5. Fabio Nunes3,
  6. Bianca Bianco1,
  7. Fernando Fonseca1,
  8. Beatriz Alves1
  1. 1 Laboratório de Análises Clínicas, Centro Universitário Saúde ABC/Faculdade de Medicina do ABC, Santo Andre, Brazil
  2. 2 Universidade Paulista, Sao Paulo, Brazil
  3. 3 Faculdade de Odontologia, Universidade de São Paulo, Sao Paulo, Brazil
  1. Correspondence to Dr Beatriz Alves, Centro Universitário Saúde ABC/ Faculdade de Medicina do ABC, Santo Andre, Brazil; bcaalves{at}uol.com.br

Abstract

Aims The aim of this study was to evaluate the mismatch repair gene expression and their correlation with NF-kB in patients with oral squamous cell carcinoma (SCC).

Methods Total RNA was isolated from 28 biopsy samples. A control group was composed of 20 volunteers. Differential expression of hMSH2, hMSH6 and NF-kB genes was accessed by quantitative PCR.

Results There is increased expression of all the analysed genes when the patients were smokers and alcoholics. In addition, there is increased expression of hMSH2 when SCC was removed from the base of the tongue. There was a correlation between NF-kB and hMSH2 and hMSH6 as well as between repair genes hMSH2 and hMSH6 expression levels. There is increased expression of the hMSH2 gene in patients with SCC, especially in the alcoholics.

Conclusions There is a strong indication that NF-kB gene was expressed along with the studied repair genes, evidencing the possibility that this system can be activated by the inflammatory pathway.

  • tumour markers
  • tumour biology
  • oncology
  • molecular biology

https://doi.org/10.1136/jclinpath-2019-206161

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    Footnotes

    • Handling editor Runjan Chetty.

    • Contributors AF and MP were responsible for the experiments and data acquisition. GV was responsible for statistical analysis. FP and FN were responsible for patient enrolment and biopsies. BB and FF were responsible for data analysis. BA and FF were responsible for conception of the work, data analysis and interpretation. All authors participated in manuscript drafting and revision.

    • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

    • Competing interests None declared.

    • Patient consent for publication Not required.

    • Ethics approval The present study was approved by the Institutional Research Ethics Committee (0094.0.017.000–11) and was conducted in full accordance with ethical principles, including the World Medical Association Declaration of Helsinki.

    • Provenance and peer review Not commissioned; internally peer reviewed.

    • Data availability statement Data are available upon reasonable request.